Human resource is the decisive factor in the development of any organization, especially service sector. Work and family domain are an increasing popular topic in the organizations and an area of concerns for human resource professionals. For many men and women today, parenthood is combined with full time paid work. The present research was to assess the work family conflict of women managers in banking industry in Thrissur district of Kerala. Even though there are various antecedents that influence the work and family conflict, this research focus on the antecedents that particularly influence the women managers in banking sector in Thrissur district. The study has highlighted that managers especially women in banking sector are having more work to family conflict than family to work conflict. The main sources of conflict in work antecedents are work involvement and work demand and in family antecedent is family demand. So, the bank management needs to be more conscious in updating their current work family balance programs.